Sensor and Probe (Request)
Identifying cell types at different neural developmental stages is the first step to study neural stem cells and developmental biology. Conventionally, cell types have been identified using antibodies which are generated mostly from rodent and requires sometimes fixation and permeabilization of cells. We are developing fluorescent imaging probes that identify specific types of cells at different developmental stages using chemicals without sacrificing cells. CDr3, Neural stem cell isolation
Pluripotent stem cells have two main categories which are embryonic and adult stem cells. These pluripotent stem cells have the function of self-renewal and multi-differentiation. We are developing fluorescent probes for different developmental stages and application methodology. CDy1, CDg4, CDb8
We have discovered three categories of probes that stain for MSC derived from various sources including omental, subcutaneous adipose tissue and bone marrow (BM). These compounds will be verified against fetal MSC, which are highly proliferative and more osteogenic than BM MSC. In stem cell bioprocessing, it is important that MSC can be produced in large quantities without senescence or loss of potency for cell therapy which require high doses and multiple injections per patient. Currently, MSC are identified by antibody based fluorescent dyes which require cell fixation. Discovering vital probes will enable live cell selection and bioprocess optimisation. Therefore, We will refine this screen using highly proliferative fetal MSC which are Stro-1 +ve and senescent MSC of very late passage.
To develop molecular fluorescence imaging probes which are capable of selective staining of pancreatic beta cell of islet. Using these probes noninvasively, we will be able to distinguish healthy islets, measure the beta cell mass of islet and reflect insulin response from both beta cell and islet itself. it would significantly aid in diabetes research both clinical and experimental approach. They can also be developed as optical probes for in vivo pancreas imaging for assessing islet delivery, the metabolic effects on the islet grafts as well as detection of immunorejection. Alpha cell, Beta cell
Peripheral blood as well as most of the organs have diverse immune cells having specific immune function against the corresponding antigen or harmful stimuli in the body. Immune cell staining and detection are useful tools for imaging of inflammation area due to the presence of the huge numbered of localized and migrating immune cells. Herein, we are developing fluorescent probes for inflammation imaging using different immune cell detections methodologies, which can be used for diagnosis or pre diagnosis.